NA 057

DIN Standards Committee Food and Agricultural Products

Project

Foodstuffs - Detection of food allergens by molecular biological methods - Part 9: Fish - Qualitative detection of a specific DNA sequence in food by real-time PCR; German and English version prEN 15634-9:2025

Abstract

This document specifies a method for the qualitative detection of fish DNA in food, of both animal and plant origin, using real-time PCR based on the Hoxc13 gene, in the context of allergen analyses. This document does not apply to representatives of the genus of cartilaginous fish (Chondrichthyes), such as sharks or rays. It is also not applicable for differentiating between fish species. The method was previously validated in an interlaboratory study (ring trial). The limit of detection of the fish real-time PCR has been determined experimentally to be at least 50 mg fish fresh weight/kg.

Begin

2025-02-27

WI

00275391

Planned document number

DIN EN 15634-9

Project number

05702776

Responsible national committee

NA 057-01-05 AA - Food allergens  

Responsible european committee

CEN/TC 275/WG 12 - Food allergens  

Contact

Nele Märtens

Am DIN-Platz, Burggrafenstr. 6
10787 Berlin

Tel.: +49 30 2601-2368
Fax: +49 30 2601-42368

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