DIN Standards Committee Food and Agricultural Products
DIN ISO 22634
Cigarettes - Determination of benzo[a]pyrene in cigarette mainstream smoke - Method using gas chromatography/mass spectrometry (ISO 22634:2008)
Zigaretten - Bestimmung von Benzo[a]pyren im Hauptstromrauch von Zigaretten - Gaschromatographisches/Massenspektrometrisches Verfahren (ISO 22634:2008)
Overview
The International Standard ISO 22634:2008 has been adopted in this standard without modification. ISO 22634:2008 has been prepared by Technical Committee ISO/TC 136 "Tobacco and tobacco products" (secretariat: DIN, Germany). The collaboration of DIN for the field of responsibility of ISO/TC 126 is performed through Working Committee NA 057-04-01 AA "Tabak und Tabakerzeugnisse" ("Tobacco and tobacco products") of the Food and Agricultural Products Standards Committee (NAL). This committee decided to adopt ISO 22634:2008 into the German body of standards. Between 1999 and 2003, a task force composed of CORESTA (Cooperation Centre for Scientific Research Relative to Tobacco) members has studied the existing methodologies for the determination of benzo[a]pyrene (B[a]P) in the mainstream smoke of cigarettes. Several methods have been proposed for this determination, which are mainly based on two types of analytical methodology: HPLC with fluorescence detection and GC-MS. In both cases, it is necessary to purify the smoke condensate extract before performing the chromatography in order to obtain a correct separation of the B[a]P peak. The task force decided in the first instance to develop a method using HPLC with fluorescence detection. However, after several collaborative experiments it appeared that achieving a significant reduction of the initially observed variability would be technically very difficult. The task force then decided to investigate a GC-MS method as an alternative and was able to demonstrate through collaborative experiments that a lower variability can be obtained with this methodology. This International Standard, produced through collaborative experiments involving many laboratories in many countries, provides an optimized procedure for the determination of benzo[a]pyrene in cigarette mainstream smoke. The repeatability and reproducibility of this method have been assessed according to ISO recommendations and are included in this standard. No machine smoking regime can represent all human smoking behaviours: - it is recommended that cigarettes also be tested under conditions of a different intensity of machine smoking than those specified in this International Standard; - machine smoking testing is useful to characterize cigarette emissions for design and regulatory purposes. However, communication of machine measurements to smokers can result in misunderstandings about differences in exposure and risk across brands; - smoke emission data from machine measurements may be used as inputs for product hazard assessment, but they are not intended to be nor are they valid measures of human exposure or risks. Communicating differences between products in machine measurements as differences in exposure or risk is a misuse of testing using ISO standards. This International Standard specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter of cigarette mainstream smoke. This method is specified using ISO 3308 smoking parameters, but is technically compatible with other smoking regimes. The method is consists of the following steps: - sampling of the test cigarettes, - conditioning of the test cigarettes, - smoking of the test cigarettes according to the smoking procedure specified in ISO 4387, - extraction of the total particulate matter, collected on the glass-fibre filter pad, with methanol, - dilution of the methanol extract with water, - elution of the water/methanol solution through a Cyclohexyl Solid Phase Extraction (CH SPE) cartridge, followed by the elution of B[a]P with cyclohexane, - analytical determination of B[a]P by gas chromatography/mass spectrometry. The committee responsible for this standard is Working Committee NA 057-04-01 AA "Tabak und Tabakerzeugnisse" ("Tobacco and tobacco products") at DIN.
Document: references other documents
Responsible national committee
NA 057-04-01 AA - Tobacco and tobacco products
