NA 057

DIN Standards Committee Food and Agricultural Products

Project

Foodstuffs - Detection of food allergens by molecular biological methods - Part 7: Peanut (Arachis hypogaea) - Qualitative detection of a specific DNA sequence in food by real-time PCR

Abstract

This document specifies a method for the qualitative detection of peanut (Arachis hypogaea) DNA in food using real-time PCR and targeting a multicopy mitochondrial sequence, in the context of allergen analyses. The method was previously validated in an interlaboratory study (ring trial) and applied to DNA extracted from samples that consist of defined proportions of peanut in rice biscuits, wheat biscuits, cooked sausage and milk powder. The limit of detection of the peanut real-time PCR has been determined experimentally to be at least 0,5 mg peanut/kg.

Begin

2025-02-20

WI

00275389

Planned document number

prEN 15634-7

Responsible national committee

NA 057-01-05 AA - Food allergens  

Responsible european committee

CEN/TC 275/WG 12 - Food allergens  

draft standard

Foodstuffs - Detection of food allergens by molecular biological methods - Part 7: Peanut (Arachis hypogaea) - Qualitative detection of a specific DNA sequence in food by real-time PCR
2026-01
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Nele Märtens

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