DIN Standards Committee Food and Agricultural Products
Foodstuffs - Determination of saxitoxin-group toxins in shellfish - HPLC method using pre-column derivatization with peroxide or periodate oxidation; German version EN 14526:2026
Abstract
This document specifies a method for the quantitative determination of saxitoxin (STX), decarbamoyl saxitoxin (dcSTX), neosaxitoxin (NEO), decarbamoyl neosaxitoxin (dcNEO), gonyautoxin 1 and 4 (GTX1,4; sum of isomers), gonyautoxin 2 and 3 (GTX2,3; sum of isomers), gonyautoxin 5 (GTX5), gonyautoxin 6 (GTX6), decarbamoyl gonyautoxin 2 and 3 (dcGTX2,3; sum of isomers), N-sulfocarbamoyl gonyautoxin 2 and 3 (C1,2; sum of isomers) and N-sulfocarbamoyl gonyautoxin 1 and 4 (C3,4; sum of isomers) in (raw) mussels, oysters, scallops and clams. Laboratory experience has shown that this document can also be applied to other marine invertebrates and processed products of those species, however, no complete interlaboratory validation study according to ISO 5725-2 has been carried out so far. The method described was validated in an interlaboratory study and was also verified in a European Union Reference Laboratory for Marine Biotoxins (EURLMB)-performance test aiming the total toxicity of the samples. Toxins which were not available in the first interlaboratory study as dcGTX2,3 and dcNEO were validated in two additional interlaboratory studies. The lowest validated levels are given as mass fraction of toxin (free base) in µg/kg shellfish tissue and also as µmol/kg shellfish tissue and are listed in Table 1.
Begin
2023-09-29
WI
00275381
Planned document number
DIN EN 14526
Project number
05702693
Responsible national committee
NA 057-01-03-01 AK - Algal toxins
Responsible european committee
CEN/TC 275/WG 14 - Marine Biotoxins
draft standard
Foodstuffs - Determination of saxitoxin-group toxins in shellfish - HPLC method using pre-column derivatization with peroxide or periodate oxidation; German and English version prEN 14526:2024
2024-12
Order from DIN Media
