Abstract

This document gives the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA). It is applicable to the testing of foodstuffs and isolates obtained from foodstuffs for food-borne microorganisms using the polymerase chain reaction (PCR). The minimum requirements laid down in this document are intended to ensure that comparable and reproducible results are obtained in different laboratories. This document has been established for food-borne microorganisms in or isolated from food and feed matrices and is applicable to: products intended for human consumption; products for feeding animals; environmental samples in the area of food and feed production and handling; samples from the primary production stage.

Begin

2021-01-04

WI

00463097

Planned document number

DIN EN ISO 22174

Project number

05702469

Responsible national committee

NA 057-01-06 AA - Microbiology of the food chain  

Responsible european committee

CEN/TC 463/WG 1 - General requirements relating to PCR methods  

Responsible international committee

ISO/TC 34/SC 9 - Microbiology  

draft standard

Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection and quantification of microorganisms - General requirements and definitions (ISO/DIS 22174:2022); German and English version prEN ISO 22174:2022
2022-11
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previous edition(s)

Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22174:2005); German version EN ISO 22174:2005
2005-05

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Microbiology of food and animal feeding stuffs - Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22119:2011); German version EN ISO 22119:2011
2011-11

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Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for amplification and detection for qualitative methods (ISO 20838:2006); German version EN ISO 20838:2006
2006-08

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Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for sample preparation for qualitative detection (ISO 20837:2006); German version EN ISO 20837:2006
2006-08

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