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Technical Specification [CURRENT]

DIN CEN ISO/TS 18867 ; DIN SPEC 10056:2016-01
Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis (ISO/TS 18867:2015); German version CEN ISO/TS 18867:2015

Title (German)

Mikrobiologie der Lebensmittelkette - Polymerase-Kettenreaktion (PCR) zum Nachweis von pathogenen Mikroorganismen in Lebensmitteln - Nachweis von pathogenen Yersinia enterocolitica und Yersinia pseudotuberculosis (ISO/TS 18867:2015); Deutsche Fassung CEN ISO/TS 18867:2015

Procedure

VN

Overview

This Technical Specification is a test standard and specifies two horizontal methods for detection of the pathogenic bioserotypes of Y. enterocolitica and one for detection of Y. pseudotuberculosis by using real-time PCRbased methods. The described methods allow for the detection of the two pathogens in enrichments and allow the isolation of colonies. Y. pestis, the causative agent of bubonic and pneumonic plague harbours a variant of the ail gene as well and will be detected by the same primer/probe set as Y. pseudotuberculosis. However, Y. pestis is normally not associated with food. This Technical Specification is applicable to products for human consumption, animal feeding stuffs, and environmental samples. It is intended for consumer health protection. The committee responsible for this standard is NA 057-01-06-01 AK "Polymerase-Kettenreaktion zum Nachweis von Mikroorganismen" ("Polymerase chain reaction for the detection of microorganisms") at DIN.

Document: references other documents

Responsible national committee

NA 057-01-06 AA - Microbiology of the food chain  

Responsible european committee

CEN/TC 463/WG 1 - General requirements relating to PCR methods  

Edition 2016-01
Original language German
Price from 94.20 €
Table of contents

Contact

Haider Klenz

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