DIN Standards Committee Radiology
DIN EN ISO 17099
Radiological protection - Performance criteria for laboratories using the cytokinesis-block micronucleus (CBMN) assay in peripheral blood lymphocytes for biological dosimetry (ISO 17099:2024); German version EN ISO 17099:2024
Strahlenschutz - Leistungskriterien für Laboratorien, die den Zytokinese-Block-Mikrokerntest (CBMN) in peripheren Blutlymphozyten für die biologische Dosimetrie verwenden (ISO 17099:2024); Deutsche Fassung EN ISO 17099:2024
Overview
This document gives guidance on a) confidentiality of personal information for the customer and the laboratory, b) laboratory safety requirements, c) calibration sources and calibration dose ranges useful for establishing the reference dose-response curves that contribute to the dose estimation from CBMN assay yields and the detection limit, d) performance of blood collection, culturing, harvesting, and sample preparation for CBMN assay scoring, e) scoring criteria, f) conversion of micronucleus frequency in BNCs into an estimate of absorbed dose, g) reporting of results, h) quality assurance and quality control, and i) informative annexes containing sample instructions for customers, sample questionnaire, a microscope scoring data sheet, and a sample report. This document excludes methods for automated scoring of CBMN The responsible national standardization committee is Working Committee NA 080-00-01 AA "Dosimetrie" ("Dosimetry") at DIN Standards Committee Radiology (NAR).
Responsible national committee
Responsible european committee
CEN/TC 430 - Nuclear energy, nuclear technologies, and radiological protection
Responsible international committee
ISO/TC 85/SC 2/WG 18 - Biological and physical retrospective dosimetry
