Abstract

This document specifies a method for the selective enumeration of bifidobacteria in milk products by using a colony-count technique at 37 °C under anaerobic conditions. The method is applicable to milk products, such as fermented (e.g. yoghurts) and non-fermented milks (e.g. pasteurized milks, skim milks, whey protein concentrates), milk powders and formulae (e.g. infant formulae, follow-up formulae for older infants, products for young children) where these microorganisms are present and viable, in combination with other lactic acid bacteria or alone. The method is also applicable to starter and probiotic cultures. For proposed quality criteria of dairy products, see, for example, CXS 243-2003. Bifidobacteria used in milk products usually belong to the following species (e.g. References [7] and [10]): - Bifidobacterium adolescentis; - B. animalis subsp. animalis; - B. animalis subsp. lactis; - B. bifidum; - B. breve; - B. longum subsp. infantis; - B. longum subsp. longum.

Begin

2025-08-13

WI

00302109

Planned document number

DIN EN ISO 29981

Project number

05702831

Responsible national committee

NA 057-05-13 AA - Milk and milk products - Methods of sampling and analysis  

Responsible european committee

CEN/TC 302 - Milk and milk products - Methods of sampling and analysis  

Responsible international committee

ISO/TC 34/SC 5 - Milk and milk products  

draft standard

Milk products - Enumeration of bifidobacteria - Colony-count technique (ISO 29981:2024); German and English version prEN ISO 29981:2025
2025-11
Order from DIN Media