Foodstuffs - Detection of food allergens by molecular biological methods - Part 7: Peanut (Arachis hypogaea) - Qualitative detection of a specific DNA sequence in food by real-time PCR; German and English version prEN 15634-7:2025
Abstract
This document specifies a for the qualitative detection of peanut (Arachis hypogaea) DNA in food using real-time PCR and targeting a multicopy mitochondrial sequence, in the context of allergen analyses. The method was previously validated in an interlaboratory study (ring trial) and applied to DNA extracted from samples that consist of defined proportions of peanut in rice biscuits, wheat biscuits, cooked sausage and milk powder. The limit of detection of the peanut real-time PCR has been determined experimentally to be at least 0,5 mg peanut/kg.
Begin
2025-02-27
WI
00275389
Planned document number
DIN EN 15634-7
Project number
05702775
Responsible national committee
NA 057-01-05 AA - Food allergens
