Abstract

This document specifies the general requirements and provides guidance for the development and application of loop-mediated isothermal amplification (LAMP) to detect microorganisms and associated genetic markers (e.g. antimicrobial resistance genes, virulence genes) in the food chain. This document is applicable to all LAMP methods, platforms, items from food chain and laboratories. The use of LAMP for quantification is out of scope for this document. Validation and verification of LAMP methods as either alternative or reference methods are not covered in this document. Both validation and verification of microbiological methods are discussed in detail in the ISO 16140 series and ISO 17468. General requirements for isothermal methods including LAMP for molecular biomarker analysis are covered by ISO 22942-1, and general requirements and definitions for polymerase chain reaction (PCR) for the detection and quantification of microorganisms in the food chain are covered by ISO 22174.

Begin

2024-03-05

WI

00463112

Planned document number

DIN EN ISO 24914

Project number

05702721

Responsible national committee

NA 057-01-06 AA - Microbiology of the food chain  

Responsible european committee

CEN/TC 463 - Microbiology of the food chain  

Responsible international committee

ISO/TC 34/SC 9/WG 36 - General requirements relating to LAMP-based methods  

draft standard

Microbiology of the food chain - Loop-mediated isothermal amplification (LAMP) for the detection of microorganisms and associated genetic markers - General requirements and definitions (ISO/DIS 24914:2025); German and English version prEN ISO 24914:2025
2025-05
Order from DIN Media