Abstract

This document gives guidance for the detection and identification of the specified microorganism Escherichia coli in technical and formulated plant biostimulants, both in liquid and solid states, and also the horizontal method for the enumeration of ß-glucuronidase-positive E. coli in plant biostimulants (both in liquid and solid states). The qualitative method described in this document is based on the detection of E. coli in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar. Other methods can be appropriate, depending on the level of detection required. The quantitative method described in this document uses a colony-count technique at 44 °C ± 1 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme ß-glucuronidase. This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of the following component EU fertilizing products categories: Fertilizers, Liming Materials, Soil Improvers, Growing Media, Plant Biostimulants, and where the following category Plant Biostimulants is the highest % in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant Biostimulants is not the highest % in the blend, the European Standard for the highest % of the blend applies. In case a blend of fertilizing products is composed of components in equal quantity or in case the component EU fertilising products used for the blend have identical formulations , the user decides which standard to apply.

Begin

2022-03-10

WI

00455027

Planned document number

DIN EN 17716

Project number

05702229

Responsible national committee

NA 057-03-06 AA - Plant biostimulants  

Responsible european committee

CEN/TC 455/WG 3 - Pathogenic and non-pathogenic microorganisms  

draft standard

Plant biostimulants - Determination of Escherichia coli; German and English version prEN 17716:2023
2023-06
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previous edition(s)

Plant biostimulants - Determination of Escherichia coli; German version CEN/TS 17716:2022
2022-07

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