NA 119

DIN-Normenausschuss Wasserwesen (NAW)


Call for interlaboratory trial for validation of ISO 10872 - Nematoda

Ankündigung für einen Ringversuch zu ISO 10872 (Deadline: 2019-08-05)

ISO 10872 Water and soil quality — Determination of the toxic effect of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans (Nematoda)

Within ISO/TC 147/SC 5 (water quality; biological methods; toxicity to invertebrates) and ISO/TC 190/SC 4/WG 2 (soil quality; biological characterization; effects on soil fauna), a ring test will be carried out in August/September 2019 for the future ISO 10872.

This International Standard specifies a method for determining the toxicity of environmental samples on growth, fertility and reproduction of Caenorhabditis elegans. The method described is applicable to whole fresh water sediment, soil and waste, as well as to pore water, elutriates and aqueous extracts, that were obtained from sediment, soil, and waste. Juvenile organisms of the species C. elegans are exposed to the environmental sample over a period of 96 h. In the control treatments the test organisms are able to complete a whole life cycle within this period. A toxic effect of an environmental sample occurs if the inhibition of growth, fertility or reproduction of C. elegans in comparison to a control (aqueous medium, control sediment or soil) exceeds a certain threshold value. Toxicity can be quantified by the intensity of the effect as percent inhibition.

The method for exposing C. elegans in soil samples has been changed in the revised version of ISO 10872. The water content of the tested soil in the test system has been reduced to 80 percent of WHCmax. This technical change requires a ring test carried out with soil samples.
In this ring test the toxicity of two soil samples (polluted soils) will be tested.
In detail, following samples will be sent between 2019-08-11 and 13:
- 2 soil samples (dry)
For the soil samples and the control soil (Lufa St. 2.2), six replicates have to be set up and all toxicity parameters (growth, fertility, reproduction) have to be analysed.

Along with the soil samples, the reference chemical BAC-16 has to be tested at a single concentration (15 mg/l) in aqueous medium. According to ISO/DIS 10872, only growth has to be analysed in the control and the BAC-16 treatment.

It is planned to publish the results of the ring test in an international peer-reviewed journal.

Interested laboratories that are experienced with toxicity tests (i.e. sediment or soil toxicity tests) are kindly asked to contact

Dr. Sebastian Höss
Giselastr. 6
82319 Starnberg
Phone: +49 8151 5509172
as soon as possible, but not later than 2019-08-05.

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